Test ID: S-141 | Sample Type: Saliva | Technology: LC-MS/MS | Results Time: 3–5 Working Days After Laboratory Receives Sample | Laboratory: ZRT Laboratory – CLIA Certified | Hormone Specialist Doctor Interpretation: Included | Suitable For: Adults and Children | Collect Sample At Home: No Clinic Visit Required
What Does This Test Measure?
Saliva — Comprehensive Male Hormone Panel (LC-MS/MS): Testosterone, Estradiol (E2), DHEAS, Progesterone, Morning Cortisol, Noon Cortisol, Evening Cortisol, Night Cortisol.
8 markers — combining the core androgenic and adrenal assessment with a full 4-point diurnal cortisol curve, revealing how HPA axis function, stress, and adrenal reserve are shaping testosterone output, recovery, and overall hormonal regulation throughout the day.
Related Symptoms
Low libido or erectile dysfunction, persistent fatigue or low energy, loss of muscle mass or poor gym recovery, increased body fat particularly around the abdomen or chest, poor sleep or night waking, afternoon energy crashes, evening anxiety or racing thoughts, low mood, brain fog, reduced motivation, burnout or overtraining, or symptoms of low testosterone with normal standard blood test results.
Did You Know?
The combination of DHEAS and a 4-point cortisol curve in a single panel reveals the full adrenal picture that neither marker alone can provide. DHEAS reflects cumulative adrenal reserve — the long-term capacity to produce androgens and oestrogens from the adrenal glands. The 4-point cortisol curve reveals the daily HPA axis rhythm — where cortisol is elevated or deficient and when. Together they identify whether adrenal dysfunction is driving androgenic decline from chronically depleted DHEAS, from cortisol-driven pregnenolone steal at specific times of day, or from both simultaneously — a clinical distinction that changes the appropriate intervention entirely.
Who Should Consider This Test?
- Low libido or reduced sexual interest
- Erectile dysfunction or reduced sexual performance
- Persistent fatigue or low energy despite adequate sleep
- Loss of muscle mass or difficulty building strength
- Increased body fat, especially around the abdomen or chest
- Brain fog, poor concentration, or reduced motivation
- Low mood, irritability, anxiety, or depression
- Poor sleep quality, night waking, or insomnia
- Afternoon energy crashes or mid-day fatigue
- Evening anxiety, restlessness, or difficulty switching off
- Symptoms of andropause, typically from age 35+
- Burnout, overtraining, or sustained high physical or psychological stress
- Plateaued training performance or poor recovery despite adequate rest
- Currently on TRT and wanting to monitor estradiol conversion and full adrenal rhythm
- Feeling hormonally off but told results are normal on standard blood tests
Why This Test Is Particularly Important for Men
- The 4-point cortisol curve reveals the full HPA axis pattern — elevated noon cortisol drives visceral fat and insulin resistance; elevated evening cortisol suppresses progesterone and testosterone precursor synthesis; elevated night cortisol directly suppresses the deep sleep phases responsible for the majority of daily testosterone production
- DHEAS combined with the full cortisol curve reveals whether adrenal depletion is acute (cortisol-mediated, affecting specific time points) or cumulative (reflected in reduced DHEAS baseline) — a distinction invisible from either marker alone
- Saliva LC-MS/MS measures free, bioavailable testosterone and estradiol — the fractions actually reaching cells; a man with high SHBG can have normal total blood testosterone but critically low free testosterone with full androgenic deficiency symptoms
- Estradiol must be measured alongside testosterone — excess estradiol from aromatase-driven conversion suppresses HPG axis testosterone output, causes gynaecomastia, and drives water retention
- Progesterone is the natural aromatase inhibitor in men — low progesterone allows unchecked testosterone-to-estradiol conversion and is frequently the hidden driver of oestrogen dominance symptoms in men with plausible testosterone values
Not Sure Which Test Is Right for You?
Email us at info@hormonelab.co.uk with your gender, age, symptoms, medications, and any current treatments. We will forward your enquiry to our doctors and respond within 24 hours.
How It Works
- Order your kit — delivered directly to your door
- Collect four saliva samples across the day — morning (7–9am, before eating or drinking), noon, evening, and night — following the detailed instructions provided
- Post your samples to ZRT Laboratory using the return instructions provided
- Receive your full report by email within 3–5 working days
What You Receive
- Testosterone, Estradiol (E2), DHEAS, Progesterone, and 4-point Cortisol results with male-specific reference ranges
- Full diurnal cortisol curve graph
- Testosterone-to-estradiol ratio, cortisol-to-testosterone ratio, and adrenal reserve assessment by a Hormone Specialist PhD Doctor
- Personalised next-step recommendations
- Laboratory fee included — no hidden charges
Why We Use ZRT Laboratory
All testing is performed by ZRT Laboratory — a globally recognised, CLIA-certified diagnostic laboratory founded in 1998 by Dr. David Zava, PhD. ZRT has performed over 11 million tests and is trusted by healthcare professionals in 96+ countries.
- Pioneers of at-home dried blood spot (DBS) and saliva hormone testing
- Advanced LC-MS/MS technology for clinical-grade accuracy
- Qualified for the CDC Hormone Standardization (HoSt) Program
- Personalised reference ranges based on age and sex
Through our partnership with ZRT Laboratory, Hormone Lab UK delivers internationally recognised, research-backed hormone testing to people across the UK and Europe — from the comfort of home.
Frequently Asked Questions
What hormones does this test measure?
Testosterone, Estradiol (E2), DHEAS, Progesterone, and Cortisol at four time points (morning, noon, evening, night) — 8 markers combining core androgenic and adrenal assessment with the full daily cortisol curve.
When should I collect my samples?
Four saliva samples are collected across the day: morning (ideally 7–9am, before eating or drinking), noon, evening, and night. Full collection instructions are included in your kit.
How does this differ from Male Saliva Hormone Profile I?
Profile I measures testosterone, estradiol, DHEAS, progesterone, and a single morning cortisol — 5 markers. The Comprehensive Profile replaces single-point cortisol with a full 4-point diurnal cortisol curve, providing complete visibility of how cortisol behaves throughout the day and its interaction with testosterone synthesis, adrenal reserve, and recovery.
Can I use this test if I am on TRT?
Yes. This test is particularly valuable for men on TRT wanting to monitor estradiol conversion and assess how the full cortisol rhythm and DHEAS reserve are interacting with testosterone therapy.
Does the price include the laboratory fee?
Yes. The full laboratory analysis fee is included. There are no additional charges. You are only responsible for posting your samples to the laboratory.
How long is the kit valid for?
The kit must be used within 12 months of the purchase date.
