Understanding Saliva Hormone Testing: Why and How It's Done

Publicado por Behcet Bicakci en


In the realm of healthcare diagnostics, testing for various hormonal levels holds significant importance in understanding an individual's health. Traditionally, blood testing has been the norm for assessing hormone levels. However, the emergence of saliva testing has brought about a new dimension in evaluating hormones. Saliva testing provides a non-invasive and convenient method for individuals to collect their samples, allowing for a more accurate representation of bioavailable hormones – those readily accessible to target tissues.

The Significance of Saliva Testing

Saliva testing measures the unbound, bioavailable fraction of hormones, which stands in contrast to blood tests that often reflect total hormone levels, including the bound, inactive fraction. This unbound fraction in saliva represents the hormones that can exert their physiological effects in the body. For instance, when assessing cortisol, the stress hormone, saliva testing can better indicate the body's stress response by capturing the free cortisol levels.

One of the primary advantages of saliva testing lies in its ability to cater to specific symptoms related to hormone imbalances. This makes it a valuable tool in monitoring hormone therapy and gauging the effectiveness of treatments.

Advancements in Saliva Hormone Testing

ZRT Laboratory, a pioneering institution in this field, has significantly contributed to the advancements in saliva hormone testing. Employing liquid chromatography/tandem mass spectrometry (LC-MS/MS), ZRT has developed more comprehensive and accurate tests for measuring a broad range of bioavailable hormones and hormone metabolites. This methodology enables precise detection of hormones even at extremely low levels, offering insights into diverse populations, including men, children, and individuals under specific medication.

Best Practices and Innovations

ZRT Laboratory emphasizes several key practices and innovations that set its saliva testing apart:

1. Single Morning Sample:

ZRT recommends collecting saliva samples in the morning as it represents the peak of daily hormone production, providing the most optimal time for measurement.

2. Ranges Adjusted for Supplementation:

Unlike some other laboratories, ZRT offers ranges adjusted for age, menstrual status, and types of supplementation, eliminating the need for patients to discontinue hormone supplementation before testing.

3. Extraction Process:

ZRT is unique in its use of an extraction process for saliva testing, ensuring accurate results, especially for low-concentration hormones such as estradiol.

4. Industry Standards:

ZRT initiated and manages the Saliva Proficiency Inter-laboratory Testing Program, ensuring high standards and accuracy across the industry.

Diverse Testing Options

ZRT Laboratory offers an extensive array of saliva hormone test kits catering to different health concerns and requirements:

  • Saliva Hormone Test Kits: Ranging from basic hormone profiles to comprehensive panels including estrogen, progesterone, testosterone, and cortisol.

  • Adrenal Stress Hormone Test Kit: Specifically focused on measuring hormones related to stress response.

  • Weight Management Hormone Test Kit: Targeting hormones associated with weight management along with thyroid and cardiovascular markers.

  • Neurotransmitter Test Kit: Evaluating neurotransmitter levels alongside saliva steroids for a more comprehensive neuro-hormonal assessment.

  • Cortisol Awakening Response Test Kit: Focused on measuring cortisol levels during the morning awakening response.


Saliva hormone testing stands as a revolutionary method offering nuanced insights into hormonal health. With its non-invasive nature, accurate assessments, and comprehensive test panels, it has emerged as a pivotal tool in the realm of personalized healthcare. ZRT Laboratory's commitment to innovation and setting high industry standards has played a pivotal role in making saliva hormone testing accessible and reliable for both healthcare providers and patients worldwide.

For individuals seeking a more precise understanding of their hormonal health or healthcare professionals aiming for comprehensive diagnostic tools, saliva hormone testing from ZRT Laboratory presents a valuable and effective solution.

Related Tests

Saliva Test Kit Includes:

Saliva Profile I – E2, Pg, T, DS & Cx1

Saliva Profile II – E2, Pg, T, DS & Cx2 

Saliva Profile III – E2, Pg, T, DS & Cx4 (Sample Report)

Hormone Trio – E2, Pg & T

Diurnal Cortisol – Cx4

Saliva + Blood Test Kit Includes:

Female Comprehensive Profile I – E2, Pg, T, DS & Cx4 (saliva); TSH, fT3, fT4 & TPOab (blood spot) (Sample Report)

Female Comprehensive Profile II – Cx4 (saliva); E2, Pg, T, SHBG, DS, TSH, fT3, fT4 & TPOab (blood spot) 

Male Comprehensive Profile I – E2, T, DS & Cx4 (saliva); PSA, TSH, fT3, fT4 & TPOab (blood spot) 

Male Comprehensive Profile II – Cx4 (saliva); E2, T, DS, SHBG, PSA, TSH, fT3, fT4 & TPOab (blood spot) 

Weight Management Test Kit Includes:

Weight Management – E2, Pg, T, Cx4 (saliva); TSH, D2, D3, In, HbA1c (blood spot)

Weight Management + Thyroid – E2, Pg, T, DS, Cx4 (saliva); TSH, D2, D3, In, HbA1c, fT3, fT4, TPOab (blood spot)

Weight Management + Cardio – E2, Pg, T, DS, Cx4 (saliva); TSH, D2, D3, In, HbA1c, hsCRP, TG, CH, LDL, VLDL, HDL (blood spot)

Weight Management + Thyroid + Cardio – E2, Pg, T, DS, Cx4 (saliva); TSH, D2, D3, In, HbA1c, fT3, fT4, TPOab, hsCRPTG, CH, LDL, VLDL, HDL

Neurotransmitters Test Kit Includes:

Neurotransmitters + Saliva Steroids  GABA, Glu, Gly, DA, Epi, NE, HIST, 5-HT, PEA, DOPAC, HVA, 5-HIAA, NMN, VMA, Trp, Kyn, 3-OHKyn, Tau, Gln, His, N-MeHist, Tyra, KynAc, Xanth, Tyr, Crtn (dried urine); E2, Pg, T, DS, C (saliva)

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