Test ID: S-125 | Sample Type: Saliva | Technology: LC-MS/MS | Results Time: 3–5 Working Days After Laboratory Receives Sample | Laboratory: ZRT Laboratory – CLIA Certified | Hormone Specialist Doctor Interpretation: Included | Suitable For: Adults and Children | Collect Sample At Home: No Clinic Visit Required
What Does This Test Measure?
Saliva — Advanced Male Hormone Panel (LC-MS/MS): Testosterone, DHEAS, Progesterone, Cortisol, Estrone (E1), Estradiol (E2), Estriol (E3).
7 markers — the most complete single-point oestrogen assessment available in a male saliva panel, combining the full oestrogen triad with androgenic status, adrenal reserve, and morning cortisol in a single at-home kit.
Related Symptoms
Low libido or erectile dysfunction, persistent fatigue or low energy, loss of muscle mass or poor gym recovery, increased body fat particularly around the abdomen or chest, poor sleep or night waking, low mood, anxiety, brain fog, reduced motivation, gynaecomastia or breast tenderness, water retention, burnout or chronic stress, or symptoms of oestrogen excess with normal total testosterone on standard blood results.
Did You Know?
Most male hormone panels measure only estradiol (E2) — the most potent oestrogen. This profile adds estrone (E1) and estriol (E3), providing the complete oestrogenic picture. Estrone is the primary oestrogen produced by adipose tissue and is the dominant oestrogen in men with higher body fat or elevated aromatase activity. Estriol provides context on overall oestrogenic load. The E1:E2:E3 ratio allows a clinically meaningful assessment of oestrogen source and metabolic conversion pathway — a level of detail that is impossible from E2 measurement alone, and that directly informs whether aromatase inhibition, progesterone support, or DHEAS optimisation is the appropriate clinical priority.
Who Should Consider This Test?
- Low libido or reduced sexual interest
- Erectile dysfunction or reduced sexual performance
- Persistent fatigue or low energy despite adequate sleep
- Loss of muscle mass or difficulty building strength
- Increased body fat, especially around the abdomen or chest
- Brain fog, poor concentration, or reduced motivation
- Low mood, irritability, anxiety, or depression
- Poor sleep quality or difficulty staying asleep
- Symptoms of andropause, typically from age 35+
- Gynaecomastia, breast tenderness, or water retention (signs of elevated oestrogenic activity)
- Burnout or sustained high psychological or physical stress
- Currently on TRT and wanting a full oestrogen triad assessment alongside androgenic and adrenal markers
- Feeling hormonally off but told results are normal on standard blood tests
Why This Test Is Particularly Important for Men
- The full oestrogen triad (E1, E2, E3) reveals oestrogen source and metabolic conversion in men — estrone elevation relative to estradiol indicates adipose-driven aromatisation; the E1:E2 ratio guides whether dietary, lifestyle, or pharmacological aromatase management is appropriate
- Saliva LC-MS/MS measures free, bioavailable testosterone and estradiol — the fractions actually reaching cells; a man with high SHBG can have normal total blood testosterone but critically low free testosterone with full androgenic deficiency symptoms, while simultaneously showing elevated free estradiol driving oestrogen dominance symptoms
- DHEAS is the primary adrenal testosterone precursor in men — its inclusion reveals whether adrenal depletion is contributing to androgenic decline and whether DHEAS supplementation may be an appropriate adjunct to direct testosterone support
- Progesterone is the natural aromatase inhibitor in men — low progesterone allows unchecked testosterone-to-estradiol conversion; its measurement alongside the full oestrogen triad identifies whether aromatase dysregulation is progesterone-mediated
- Cortisol provides the HPA axis context — elevated morning cortisol drives pregnenolone steal, suppresses DHEAS production, and promotes visceral fat-driven aromatase activity; this pattern is the most common adrenal mechanism behind oestrogen dominance in men
Not Sure Which Test Is Right for You?
Email us at info@hormonelab.co.uk with your gender, age, symptoms, medications, and any current treatments. We will forward your enquiry to our doctors and respond within 24 hours.
How It Works
- Order your kit — delivered directly to your door
- Collect your saliva sample in the morning (ideally 7–9am), when testosterone is naturally at its peak, before eating, drinking, or brushing your teeth
- Post your sample to ZRT Laboratory using the return instructions provided
- Receive your full report by email within 3–5 working days
What You Receive
- Testosterone, DHEAS, Progesterone, Cortisol, Estrone (E1), Estradiol (E2), and Estriol (E3) results with male-specific reference ranges
- Visual charts for all seven hormones
- Full oestrogen triad ratio analysis, testosterone-to-estradiol assessment, and adrenal–androgenic interaction evaluation by a Hormone Specialist PhD Doctor
- Personalised next-step recommendations
- Laboratory fee included — no hidden charges
Why We Use ZRT Laboratory
All testing is performed by ZRT Laboratory — a globally recognised, CLIA-certified diagnostic laboratory founded in 1998 by Dr. David Zava, PhD. ZRT has performed over 11 million tests and is trusted by healthcare professionals in 96+ countries.
- Pioneers of at-home dried blood spot (DBS) and saliva hormone testing
- Advanced LC-MS/MS technology for clinical-grade accuracy
- Qualified for the CDC Hormone Standardization (HoSt) Program
- Personalised reference ranges based on age and sex
Through our partnership with ZRT Laboratory, Hormone Lab UK delivers internationally recognised, research-backed hormone testing to people across the UK and Europe — from the comfort of home.
Frequently Asked Questions
What hormones does this test measure?
Testosterone, DHEAS, Progesterone, Cortisol, Estrone (E1), Estradiol (E2), and Estriol (E3) — 7 markers providing the most complete male oestrogen and androgenic panel available from a single at-home saliva test.
When should I collect my sample?
Collect in the morning, ideally between 7–9am, when testosterone is naturally at its daily peak. Full collection instructions are included in your kit.
How does this differ from Male Saliva Hormone Profile I?
Profile I measures testosterone, estradiol, DHEAS, progesterone, and morning cortisol — 5 markers. Profile III extends this by adding estrone (E1) and estriol (E3), providing the complete oestrogen triad for a full assessment of oestrogenic source and metabolic conversion in men.
Can I use this test if I am on TRT?
Yes. This test is particularly valuable for men on TRT wanting the most complete oestrogen picture alongside androgenic and adrenal markers to guide aromatase management and supplementation decisions.
Does the price include the laboratory fee?
Yes. The full laboratory analysis fee is included. There are no additional charges. You are only responsible for posting your sample to the laboratory.
How long is the kit valid for?
The kit must be used within 12 months of the purchase date.
