Advanced Neurotransmitter & Hormone Test II | 50 Markers

Test ID: U-343 | Sample Type: Dried Urine, 4 Timed Collections | Technology: LC-MS | Laboratory: ZRT Laboratory, CLIA-certified | Specialist Laboratory Interpretation: Included | Results: 3–5 working days after the laboratory receives your samples | Suitable For: Children and Adults | Collect Sample At Home: No Clinic Visit Required

If poor sleep, daytime tiredness, feeling constantly “wired”, low motivation or brain fog are affecting everyday life, this combined test provides laboratory information to help you explore these concerns with your practitioner.

The Advanced Neurotransmitter & Hormone Test II combines the Advanced 26-marker urinary neurotransmitter profile with timed measurements of free cortisol, free cortisone, the melatonin metabolite MT6s, norepinephrine and epinephrine. Four at-home urine collections provide daily profiles alongside neurotransmitter-related results, bringing several areas together in one report.

Your results include numerical values, reference ranges, graphical daily profiles and specialist laboratory interpretation, helping you prepare a more focused discussion about your symptoms and appropriate next steps.

What Does the Combined Neurotransmitter and Hormone Test Measure?

The panel reports 50 measurements, including repeated measurements across the day.

The total comprises the Advanced 26-marker profile, 12 cortisol/cortisone/MT6s measurements, four additional norepinephrine measurements, four additional epinephrine measurements and four additional creatinine measurements.

The total includes repeated measurements, not 50 different substances. Creatinine contributes to the total as a urine-concentration marker. Five reported measurements of norepinephrine, epinephrine and creatinine do not mean five urine collections.

Neuro Advanced Profile Includes:

Additional Daily Hormone and Sample-Correction Measurements:

Norepinephrine, epinephrine and creatinine are each reported five times across the combined profile, including their entries in the Advanced profile. Collection requires four timed dried urine samples.

Not Sure Which Test Is Right for You?

Email info@hormonelab.co.uk with your age, gender, symptoms and how long you have experienced them, current medicines and supplements, existing treatments and questions for our doctors. We will forward your enquiry to our doctors and aim to respond within 24 hours with a test suggestion.

How Can This Test Help?

• Explore overlapping concerns: bring sleep, energy, mood, concentration and stress-related findings together in one report.
• See measured daily patterns: timed cortisol, cortisone, MT6s, norepinephrine and epinephrine results provide information from different points in your collection day.
• Review related markers together: urinary neurotransmitters, precursors and metabolites provide a broader set of measurements than a single-marker test.
• Make your consultation more focused: use the report and specialist interpretation to prepare questions and discuss appropriate further assessment, nutrition, lifestyle or wellbeing steps with your practitioner.
• Collect conveniently at home: four dried urine collections using the supplied cards, with no clinic visit required.

Could This Test Help You Explore Your Symptoms?

Sleep, mood, energy and concentration concerns often overlap. This profile brings urinary neurotransmitter-related results together with daily hormone, melatonin-metabolite and adrenaline-related measurements, giving your practitioner several sets of findings to review alongside your symptoms.

Low Mood, Irritability or Frequent Mood Changes

Low mood, emotional sensitivity, loss of enjoyment or increased irritability can affect relationships, motivation and everyday wellbeing.

Markers assessed include:

• Serotonin (5-HT)
• Dopamine (DA)
• Phenethylamine (PEA)
• GABA
• DOPAC
• HVA
• 5-HIAA
• Tryptophan
• Kynurenine Pathway Markers

How this test may help: Serotonin and dopamine are measured alongside their urinary metabolites and precursors. The daily hormone and MT6s profiles add information when mood concerns occur with poor sleep, tiredness or stress, providing a broader set of findings for your consultation.

Feeling Anxious, Tense or Overwhelmed

These concerns may include excessive worry, nervousness, racing thoughts, muscle tension, panic symptoms or difficulty relaxing.

Markers assessed include:

• GABA
• Glutamate
• Glycine
• Norepinephrine
• Epinephrine
• Normetanephrine
• VMA
• Cortisol
• Cortisone

How this test may help: The panel combines urinary neurotransmitter-related measurements with timed cortisol, cortisone, norepinephrine and epinephrine results. Reviewing these alongside when you feel tense or overwhelmed adds useful laboratory information to a discussion about stress, sleep and your daily routine.

Difficulty Falling Asleep, Staying Asleep or Waking Refreshed

Trouble switching off, frequent waking or feeling unrefreshed can affect both daytime energy and emotional wellbeing.

Markers assessed include:

• GABA
• Glycine
• Glutamate
• Serotonin
• Histamine
• Taurine
• 6-Sulfatoxymelatonin (MT6s)
• Cortisol
• Cortisone

How this test may help: Timed MT6s measurements provide information about urinary melatonin metabolism, while cortisol and cortisone add daily glucocorticoid profiles. Your practitioner can review these with the neurotransmitter results, your sleep diary and routine to discuss appropriate next steps.

Daytime Fatigue or Fluctuating Energy

Feeling tired throughout the day, experiencing afternoon slumps or struggling with stamina can make everyday tasks harder.

Markers assessed include:

• Dopamine
• Norepinephrine
• Epinephrine
• PEA
• Cortisol
• Cortisone

How this test may help: Timed hormone and adrenaline-related results provide measured daily patterns to review alongside the times you feel tired or more alert. The neurotransmitter profile adds information when fatigue overlaps with low motivation, poor concentration or stress.

Brain Fog or Difficulty Concentrating

Symptoms may include poor focus, forgetfulness, reduced mental clarity, slow thinking or difficulty completing tasks.

Markers assessed include:

• Dopamine
• Norepinephrine
• Glutamate
• Serotonin
• Tyrosine
• Tryptophan
• Kynurenine
• Kynurenic Acid
• 3-Hydroxykynurenine

How this test may help: These urinary compounds, precursors and metabolites provide additional laboratory information for a review of concentration concerns. Daily hormone and MT6s profiles add context when brain fog occurs alongside disturbed sleep or changing energy levels.

Low Motivation

Reduced drive, lack of enthusiasm, procrastination or loss of interest in activities can affect your work and personal life.

Markers assessed include:

• Dopamine
• DOPAC
• HVA
• PEA
• Tyrosine

How this test may help: Dopamine is measured alongside its related metabolites and the precursor tyrosine. These findings provide a broader set of laboratory information to discuss when low motivation overlaps with mood, sleep or energy concerns.

Difficulty Coping With Stress or Switching Off

Some people feel unable to unwind after stressful situations or remain mentally and physically “switched on” long after an event has passed.

Markers assessed include:

• Cortisol (4 measurements)
• Cortisone (4 measurements)
• Norepinephrine (5 measurements)
• Epinephrine (5 measurements)
• GABA
• Serotonin
• Glutamate

How this test may help: If you feel constantly switched on or struggle to unwind, the timed cortisol, cortisone, norepinephrine and epinephrine results provide measured daily patterns to review alongside when your symptoms occur. This adds useful information to a discussion about stress, sleep and your daily routine.

Why Is This Profile Different?

U-343 combines:

• The Advanced 26-marker urinary neurotransmitter profile, including creatinine
• Neurotransmitter metabolites and amino acid precursors such as tryptophan and tyrosine
• Selected tryptophan-kynurenine pathway markers
• Four timed urinary free cortisol measurements
• Four timed urinary free cortisone measurements
• Four timed urinary melatonin-metabolite (MT6s) measurements
• Additional timed norepinephrine and epinephrine measurements
• Additional creatinine measurements for urine concentration correction

Parent neurotransmitters are measured alongside related metabolites, including dopamine with DOPAC and HVA, serotonin with 5-HIAA, and histamine with N-methylhistamine. Reviewing related compounds together provides a broader biochemical picture than considering individual results alone.

Compared with the 42-marker U-342 panel, U-343 adds four further measurements each of norepinephrine and epinephrine, increasing the total to 50. Both kits use four timed dried urine collections.

Why Test Neurotransmitters and Daily Hormone Patterns Together?

The panels provide different but complementary sets of laboratory information. The neurotransmitter profile measures selected urinary compounds, precursors and metabolites. The daily profile adds timed glucocorticoid, melatonin-metabolite and catecholamine measurements.

Reviewing these together provides several areas of information within one report, particularly useful for discussion when mood or concentration concerns occur alongside sleep, energy and stress concerns.

What Are Neurotransmitters and the Hormones Included in This Panel?

Neurotransmitters are chemical messengers that help nerve cells communicate. They are involved in mood, attention, movement, sleep and stress responses. This kit measures selected neurotransmitters and related compounds excreted in urine.

Cortisol is involved in the body’s stress response and daily rhythms. Cortisone provides related information about glucocorticoid metabolism. MT6s is the main urinary breakdown product of melatonin. Norepinephrine and epinephrine have roles in nervous-system signalling and the body’s stress response.

Choosing Between Related Panels

The Advanced 26-marker kit provides the urinary neurotransmitter profile. The 42-marker U-342 kit adds timed cortisol, cortisone and MT6s. U-343 further extends this combination with timed norepinephrine and epinephrine measurements.

For a combined urinary profile with saliva hormones, consider the 49-marker SU-841 kit or the 57-marker Elite kit. The Elite kit combines seven saliva hormone measurements with the extended U-343 urinary profile.

Dried Urine Sample Collection Instructions

Collect four timed dried urine samples using the supplied materials.

Before You Begin

Read the instructions supplied with your kit. Record all current medications, supplements and hormone treatments on the Test Requisition Form.

Choosing Your Collection Day

• Men and children: Collect on any day of the month.
• Women who do not menstruate, including postmenopausal women: Collect on any day of the month.
• Women with regular periods: Choose a non-bleeding day. This urine-only test does not require collection on a specific day of your menstrual cycle.
• Women with irregular or infrequent periods: Collect on any non-bleeding day.

Avoid collecting during menstruation or whenever blood may be present in your urine. If you use hormone treatments, also follow the guidance below.

When to Collect

All groups should collect samples at the following four times:

Before your first collection, do not eat, drink anything other than water, or take vitamins or supplements. Follow your healthcare provider’s advice about medication timing; do not stop prescribed medication without their advice.

Limit fluid intake to no more than approximately 240 ml (8 oz) during the hour before each collection to avoid diluting your sample.

How to Collect Each Sample

• Remove a collection card from its bag. Write your name, collection date and time on the card before collecting.
• Open the cover and fold it away from the filter paper. Avoid touching the filter paper.
• Fully saturate the filter paper by urinating directly onto it, or collect urine in a clean cup and dip the paper up to the top black dashed line.
• Hang or tape the card somewhere secure to dry. Ensure the wet filter paper does not touch anything.
• Allow the sample to dry completely for at least six hours. Once completely dry, refold the card, place it in its bag and seal it. Do not leave samples outside the bag for longer than 24 hours.
• Repeat these steps for each collection time.

If You Use Hormone Treatments

Record all hormone treatments on your Test Requisition Form. Follow the hormone-use instructions supplied with your kit and your healthcare provider’s advice. Do not stop prescribed hormone treatment without their advice.

• Morning hormones: Take or apply your usual morning dose after the first urine collection. Wash your hands thoroughly afterwards, especially after applying topical hormones.
• Nighttime hormones: Collect your bedtime sample before taking or applying your nighttime dose.
• Vaginal hormones: The laboratory’s instructions advise avoiding vaginal hormone use during the collection day until after the final urine sample. Discuss timing with your healthcare provider if needed.

If You Miss a Collection

Collect the missed sample at the same time the following day. For example, collect a missed evening sample the following evening. Record the actual collection date and time on both the card and the Test Requisition Form, then follow the same drying and sealing instructions.

Returning Your Samples

Complete the Test Requisition Form in full. Place it, any additional paperwork and all sealed, completely dry samples into the test kit box. Pack and return the kit according to the return instructions provided.

A return envelope is provided with your kit. It is not prepaid; customers are responsible for return postage to the laboratory.

Important: Urine samples that are wet, unsealed, excessively diluted or more than 30 days old will not be processed.

Collection Instructions: View dried urine collection instructions

Advanced LC-MS Technology

The panel uses Liquid Chromatography Mass Spectrometry, or LC-MS, to analyse its urinary markers. Specialist laboratory interpretation accompanies the results.

Convenient At-Home Collection

Collect four timed dried urine samples using the supplied filter cards. No blood draw or clinic visit is required. Allow the cards to dry fully before sealing and returning them according to the kit instructions.

How the Test Works

1. Order your kit and receive the collection materials.
2. Collect four timed dried urine samples according to the supplied timetable.
3. Dry, package and return your samples using the provided instructions.
4. Receive your results by email, normally within 3–5 working days after the laboratory receives your samples.

What You Receive With Your Results

Your report covers the panel’s 50 measurements, with numerical values, laboratory reference ranges, graphical daily profiles and specialist laboratory interpretation.

Share the report with your practitioner to review the findings alongside your symptoms and routine. Keeping a note of sleep, energy and stress concerns on your collection day can help make that discussion more focused.

Kit Fees, Postage and Validity

Laboratory analysis and specialist interpretation are included. A return envelope is provided with your kit. It is not prepaid; customers are responsible for return postage to the laboratory. Use the kit within 12 months of purchase.

Follow the preparation instructions and record medicines, supplements and hormone treatments on your requisition form.

About ZRT Laboratory

All testing is performed by ZRT Laboratory, a globally recognised, CLIA-certified diagnostic laboratory founded in 1998 by Dr. David Zava, PhD. ZRT has performed over 11 million tests and is trusted by healthcare professionals in 96+ countries.

• Pioneers of at-home dried urine neurotransmitter and hormone testing
• Advanced LC-MS/MS technology for clinical-grade accuracy
• Qualified for the CDC Hormone Standardisation (HoSt) Program
• Rigorous internal and external quality assurance programmes

Hormone Lab UK (Testfield UK Ltd) is the official UK test provider for ZRT Laboratory. All samples are analysed by ZRT Laboratory, a CLIA-certified laboratory with over 25 years of experience in hormone, neurotransmitter, and wellness testing.

Frequently Asked Questions

How can this test help when sleep, mood and energy concerns overlap?

It combines urinary neurotransmitter-related results with timed cortisol, cortisone, MT6s, norepinephrine and epinephrine profiles. This gives your practitioner several sets of findings to review in one report and helps you prepare a focused discussion about next steps.

What do the additional adrenaline-related measurements add?

Additional timed norepinephrine and epinephrine results extend the daily urinary profile. These provide measured patterns to discuss alongside the times you feel wired, tense, tired or more alert.

How can the results support a discussion about sleep?

Timed MT6s results provide information about urinary melatonin metabolism. Reviewing these with cortisol, cortisone and neurotransmitter-related measurements adds laboratory information to your sleep diary and routine.

Can the report be useful when my energy changes during the day?

Timed hormone and adrenaline-related results provide daily patterns to review alongside when you feel tired or more alert. The neurotransmitter profile adds information when energy concerns overlap with brain fog, mood changes or low motivation.

Why measure cortisol and cortisone together?

They provide related but different urinary glucocorticoid measurements. Reviewing both timed profiles adds context about measured cortisol and cortisone levels across the collection day.

What is MT6s?

6-Sulfatoxymelatonin, or MT6s, is the main urinary metabolite of melatonin. Its timed measurements provide information relevant to melatonin metabolism and sleep-wake patterns.

How is the 50-measurement total calculated?

It includes the Advanced 26-marker profile, 12 timed cortisol/cortisone/MT6s measurements, four additional norepinephrine measurements, four additional epinephrine measurements and four additional creatinine measurements.

Why are norepinephrine and epinephrine listed in both tables?

Each appears once in the Advanced profile and has four additional measurements in the combined daily profile, giving five reported measurements of each. Collection requires four timed urine samples.

Why is creatinine included?

Creatinine helps account for differences in urine concentration when interpreting the results.

How many samples do I collect?

Four dried urine samples: your first urination upon waking, your second approximately two hours later, before dinner and before bedtime.

Which panel adds saliva hormones to this urinary combination?

The 57-marker Elite kit adds seven saliva hormone measurements to the extended U-343 urinary profile. The 49-marker SU-841 kit combines seven saliva hormones with the U-342 urinary profile.

Is this test suitable for children and adults?

The dried urine collection method is non-invasive and suitable for children and adults. 

Do I need to visit a clinic?

No. Collect Sample At Home: No Clinic Visit Required. Use the supplied collection cards and follow the drying and return instructions.

What if I take medicines, supplements or hormone treatments?

Record them on the requisition form and follow the kit instructions. Do not change prescribed treatment without healthcare provider advice.

What do I receive with my results?

Numerical values, laboratory reference ranges, graphical daily profiles and specialist laboratory interpretation covering the panel’s 50 measurements.

How can I use my report?

Share it with your practitioner alongside your symptoms, collection times, medicines and supplements. Use the interpretation to prepare questions about the findings and discuss appropriate further assessment or nutrition, lifestyle and wellbeing steps.

When will I receive my results?

Results are normally emailed within 3–5 working days after the laboratory receives your samples.

Are laboratory fees included?

Yes. Laboratory analysis and specialist interpretation are included.

Is the return envelope prepaid?

No. A return envelope is supplied, but customers pay return postage to the laboratory.

How long is the kit valid for?

Use the kit within 12 months of purchase.

Related Neurotransmitter and Hormone Test Panels

• Advanced Neurotransmitter Test Kit | 26 Markers
• Advanced Neurotransmitter & Diurnal Hormone Test | 42 Markers
• Advanced Neurotransmitter & Hormone Test I | 49 Markers
• Elite Neurotransmitters & Saliva Hormone Test | 57 Markers
• Neurotransmitter & Saliva Hormone Test II | 36 Markers