Male Advanced Hormone, Stress & Thyroid Health Test | Specialist Doctor Report Included

Test ID: SB-650 | Saliva LCMS Hormones 7 with 4-Point Cortisol + Blood Spot Full Thyroid Panel

The Male Advanced Hormone, Stress & Thyroid Health Test is the most clinically comprehensive at-home hormone profile available for men, measuring 16 key hormones and markers across three interconnected systems: androgenic and sex hormone balance, the full daily cortisol stress rhythm, and complete thyroid function including autoimmune markers. It combines saliva LCMS (Liquid Chromatography–Mass Spectrometry) testing for sex hormones and cortisol with a full blood spot thyroid panel — the most analytically rigorous combination available for assessing male hormonal health in its full systemic context.

This test is designed for men who want definitive answers, not partial data. In men, testosterone, oestradiol, progesterone, DHEAS, cortisol, and thyroid hormones operate as a single integrated system. Cortisol depletes testosterone through pregnenolone steal and Leydig cell suppression. Thyroid insufficiency directly impairs testosterone synthesis, reduces metabolic rate, and compounds every symptom of androgen decline. Elevated oestradiol from visceral fat suppresses testosterone through aromatase while simultaneously increasing cardiovascular risk. And DHEAS — the adrenal androgen reserve — declines from the mid-30s, eroding the hormonal foundation that supports testosterone production, stress resilience, and metabolic function. This test measures all three systems together in a single at-home kit, providing a level of clinical insight that standard NHS blood panels and single-hormone tests cannot replicate.

Test from home with confidence — no clinic visit required. Results within 3–5 working days.

Why This Is the Most Important Hormone Test a Man Can Take

No other at-home test combines LCMS sex hormone accuracy with 4-point cortisol rhythm assessment and a full autoimmune thyroid panel in a single kit. Here is why each component is essential and why they must be assessed together in men:

• Cortisol is the primary suppressor of testosterone in men — through pregnenolone steal, chronic cortisol elevation diverts the shared hormonal precursor away from testosterone synthesis; direct Leydig cell suppression further reduces testosterone production at the gonadal level; simultaneously, cortisol drives visceral fat accumulation, which increases aromatase activity and oestradiol conversion — creating a compounding hormonal deficit that TSH and total testosterone blood tests cannot detect
• Thyroid dysfunction is a major and overlooked cause of low testosterone in men — hypothyroidism directly suppresses Leydig cell function and testosterone production; cortisol-driven T3 suppression produces identical hypothyroid symptoms — fatigue, weight gain, cold intolerance, brain fog, low libido — with a normal TSH; Hashimoto’s autoimmunity is present in men years before TSH becomes abnormal; the full thyroid panel including FT3, FT4, TT4, TPO and Tg antibodies is the only way to assess thyroid function comprehensively in men
• LCMS is the gold standard for measuring bioavailable testosterone and sex hormones in men — standard immunoassay blood tests measure total testosterone including the SHBG-bound, inactive fraction; saliva LCMS measures the free, bioavailable fraction that actually reaches cells, drives sexual function, energy, and muscle anabolism, and correlates with symptoms; in men with elevated SHBG — from ageing, thyroid dysfunction, or low insulin — total testosterone can appear normal while free testosterone is critically low
• Oestradiol balance is essential for male health but easily disrupted — men need oestradiol for bone density, cardiovascular protection, and cognitive function; but excess oestradiol — from aromatase activity in visceral fat, cortisol-driven SHBG elevation, or alcohol — suppresses testosterone, causes gynaecomastia, drives water retention, and raises cardiovascular risk; measuring all three oestrogens reveals not just oestradiol level but the full oestrogenic load and conversion pathway
• DHEAS is the adrenal androgen reserve that sustains testosterone under stress — DHEAS is the primary precursor for peripheral testosterone and oestrogen synthesis; declining DHEAS from the mid-30s reduces the hormonal buffer available during stress, illness, and overtraining; low DHEAS is associated with fatigue, reduced testosterone availability, poor recovery, low libido, and accelerated hormonal ageing; without measuring DHEAS, the adrenal contribution to androgenic health is invisible

What This Test Measures

Saliva LCMS — Sex Hormones

• Oestradiol (E2): Present in men via aromatase conversion of testosterone. Critical for bone density, cardiovascular protection, and cognitive function — but excess oestradiol suppresses the hypothalamic-pituitary-gonadal axis, reduces LH signalling, causes gynaecomastia, and drives fat accumulation and water retention. Measured by LCMS for bioavailable (free) fraction accuracy — the clinically relevant value in men.
• Oestriol (E3): Reflects oestrogen conversion pathways and metabolic activity in men. The E3-to-E2 ratio provides context for how oestrogens are being metabolised — elevated E3 can indicate excessive aromatase activity or oestrogen precursor load that the E2 value alone would not reveal.
• Oestrone (E1): Produced primarily by androgen conversion in adipose tissue. Elevated oestrone in men with visceral fat or metabolic dysfunction drives systemic oestrogenic load, compounds testosterone suppression, and is associated with increased cardiovascular and metabolic risk. A key indicator of adipose-driven hormonal disruption in men.
• Progesterone (Pg): Present in men in small but clinically important amounts. Acts as a natural 5-alpha reductase inhibitor, blocking the conversion of testosterone to DHT; has direct anti-oestrogenic, neuroprotective, and anti-inflammatory effects; and supports thyroid function and sleep quality. Low progesterone in men is associated with elevated DHT, prostate risk, scalp hair loss, anxiety, and poor sleep architecture.
• Testosterone (T): The primary male androgen — responsible for energy, libido, muscle mass, bone density, cognitive performance, and mood. Saliva LCMS measures free, bioavailable testosterone — the fraction that actually drives androgenic function — providing clinically superior information to total blood testosterone, which includes the bound, inactive SHBG fraction and can mask significant free testosterone deficiency.
• DHEAS (DS): The primary adrenal androgen precursor in men, declining steadily from the mid-30s and depleted rapidly under chronic stress or overtraining. Low DHEAS reduces testosterone precursor availability, impairs stress resilience, and is associated with fatigue, reduced libido, and accelerated hormonal ageing. Elevated DHEAS under adrenal overactivation or sustained overtraining drives androgen excess and cardiovascular inflammatory risk.

Saliva — 4-Point Cortisol Profile

• Morning Cortisol: The Cortisol Awakening Response — the morning surge that activates metabolism, sets testosterone availability and LH pulsatility for the day, and establishes cognitive and physical readiness. A blunted morning response signals HPA axis burnout and impaired testosterone recovery; an exaggerated response indicates high stress reactivity and cortisol-driven testosterone suppression from the first hours of the day.
• Noon Cortisol: Elevated noon cortisol promotes insulin resistance, visceral fat accumulation, and aromatase-driven testosterone-to-oestradiol conversion in men. It simultaneously impairs thyroid T4-to-T3 conversion, reducing the active thyroid hormone available to support metabolism and testosterone synthesis in the most metabolically active part of the day.
• Evening Cortisol: Should be declining significantly by early evening to allow testosterone and growth hormone to begin rising during early sleep cycles. Elevated evening cortisol in men directly suppresses overnight hormonal recovery, elevates SHBG through hepatic cortisol-SHBG coupling, and impairs thyroid hormone cellular uptake during sleep.
• Night Cortisol: Elevated night cortisol suppresses the deep sleep phases during which the majority of daily testosterone is produced and growth hormone peaks. It promotes overnight visceral fat accumulation, blood sugar dysregulation, and aromatase-driven oestradiol excess — the combination most responsible for the compounding hormonal decline seen in chronically stressed men.

Blood Spot — Full Thyroid Panel

• TSH (Thyroid Stimulating Hormone): The standard first-line thyroid screen — but in men, TSH alone misses cortisol-driven T3 suppression, early thyroid insufficiency, and Hashimoto’s autoimmunity that may be present years before TSH becomes abnormal. A normal TSH does not exclude clinically significant thyroid dysfunction in a symptomatic man.
• Free T3 (FT3): The active thyroid hormone that drives metabolism, energy production, testosterone synthesis support, cognitive function, and body temperature in men. Low FT3 with normal TSH is the hallmark of cortisol-driven thyroid suppression — the most commonly missed hormonal pattern in men under chronic occupational or psychological stress.
• Free T4 (FT4): The thyroid’s primary output hormone, converted to active T3 in peripheral tissues. Low FT4 with normal TSH indicates early thyroid insufficiency or impaired peripheral conversion, often preceding symptomatic hypothyroidism by years.
• Total T4 (TT4): Measures total circulating T4 including the bound fraction. Provides important interpretive context for FT4, particularly relevant in men on TRT where thyroid-binding proteins may be altered.
• TPO Antibodies (Anti-TPO): The primary diagnostic marker for Hashimoto’s thyroiditis in men. Elevated TPO antibodies frequently precede abnormal TSH by years and are associated with progressive thyroid decline, fatigue, metabolic deterioration, and Leydig cell testosterone suppression.
• Thyroglobulin Antibodies (Anti-Tg): A second autoimmune marker essential for complete Hashimoto’s screening — some men have elevated Tg antibodies with normal TPO, making both markers necessary for accurate autoimmune thyroid assessment and early detection of progressive thyroid decline.

Who Should Consider This Test?

This test is the ideal choice for men who experience:

• Persistent fatigue or exhaustion that does not improve with sleep or rest
• Low libido or reduced sexual interest
• Erectile dysfunction or reduced sexual performance
• Unexplained weight gain or difficulty losing body fat, particularly around the abdomen
• Poor recovery from exercise, training, or sustained high workload
• Brain fog, poor concentration, or reduced cognitive sharpness
• Low mood, reduced motivation, or mild depression
• Feeling cold, temperature sensitivity, or slow metabolism
• Dry skin, hair thinning or loss, or slow wound healing
• Acne, oily skin, scalp hair loss, or gynaecomastia
• Insomnia, night waking, or unrefreshing sleep
• Chronic stress, burnout, or sustained high-pressure workload
• Symptoms of low testosterone with normal or inconclusive standard blood results
• Currently on TRT and wanting a comprehensive hormonal and thyroid picture
• Diagnosed or suspected Hashimoto’s thyroiditis or autoimmune thyroid disease
• Family history of thyroid disease, cardiovascular disease, or metabolic syndrome
• Men over 35 seeking the most comprehensive single at-home androgenic and thyroid health assessment available

Key Features

• 16-marker advanced triple-system panel: saliva LCMS sex hormones, 4-point cortisol, and full blood spot thyroid profile
• Saliva LCMS — the gold standard for bioavailable (free) testosterone and sex hormone measurement in men
• All three oestrogens (E1, E2, E3) for complete oestrogenic load and aromatase activity assessment
• Full thyroid panel including TPO and Tg autoimmune antibodies
• 4-point cortisol profile revealing the complete daily adrenal rhythm and its impact on testosterone and thyroid
• Results within 3–5 working days after lab receipt
• Expert analysis by a Hormone Specialist PhD Doctor
• Clear graphical and numerical results with personalised commentary
• All-inclusive price — laboratory fees included, no hidden costs
• Valid for 12 months from purchase

How It Works

1. Order the kit online — delivered directly to your door.
2. Collect your saliva samples at four time points (morning, noon, evening, night) for the sex hormone and cortisol panel, and your blood spot sample via a simple finger-prick for the thyroid panel, following the detailed instructions provided. Morning saliva should be collected before 9am for optimal cortisol accuracy.
3. Post your samples to the lab using the return instructions included in your kit.
4. Receive your results within 3–5 working days, with specialist commentary and clear next-step guidance.

What Your Report Includes

• All 16 marker results with male-specific reference ranges
• Visual charts for all six sex hormones, your full 4-point cortisol daily curve, and thyroid panel values
• Clinical interpretation of how your testosterone, oestradiol, progesterone, DHEAS, cortisol, and thyroid markers interact as an integrated male hormonal system
• Free testosterone assessment based on bioavailable LCMS measurement
• Oestradiol-to-testosterone ratio and total oestrogenic load analysis
• Cortisol-thyroid interaction assessment — including whether cortisol is impairing T3 conversion and suppressing Leydig cell function
• Specialist commentary by a Hormone Specialist PhD Doctor
• Personalised next-step recommendations

Frequently Asked Questions

What does this test measure?

Oestradiol (E2), Oestriol (E3), Oestrone (E1), Progesterone, Testosterone, DHEAS (saliva LCMS) plus 4-point Cortisol (saliva: morning, noon, evening, night) plus TSH, Free T3, Free T4, Total T4, TPO Antibodies, and Thyroglobulin Antibodies (blood spot) — 16 markers in total.

Why is LCMS used for sex hormones in this test?

LCMS (Liquid Chromatography–Mass Spectrometry) is the most analytically precise method for sex hormone measurement. It measures the free, bioavailable fraction of hormones — the portion actually reaching cells and driving function. In men, this distinction is critical: total testosterone measured by immunoassay includes SHBG-bound hormone that is biologically inactive; a man with high SHBG can have normal total testosterone but critically low free testosterone. LCMS reveals the true androgenic picture that standard blood tests miss.

Why do men need oestrogen tested?

Men produce oestradiol through aromatase conversion of testosterone — a process that increases with visceral fat, alcohol, ageing, and cortisol elevation. Oestradiol is essential for bone health, cardiovascular function, and libido in men, but excess suppresses testosterone, causes gynaecomastia, and drives fat accumulation. Measuring all three oestrogens (E1, E2, E3) alongside testosterone reveals the full oestrogenic load and aromatase activity driving hormonal imbalance in men.

Can I use this test if I am on TRT or levothyroxine?

Yes. This test is particularly valuable for men on TRT, who need to monitor oestradiol balance, cortisol pattern, and thyroid function alongside testosterone. TRT increases aromatisation, can alter thyroid binding, and is most effective when cortisol dysregulation and thyroid insufficiency are identified and addressed. Follow the instructions provided in your kit.

How accurate is the LCMS hormone testing?

LC-MS/MS (Liquid Chromatography–Tandem Mass Spectrometry) is the reference standard for hormone analysis, used by national reference laboratories and research institutions worldwide. It provides superior specificity and sensitivity compared to immunoassay methods, particularly for testosterone and oestrogens where cross-reactivity in immunoassays can produce inaccurate results.

When will I get my results?

Within 3–5 working days after your samples reach the laboratory.

Are there hidden costs?

No. Laboratory analysis and specialist review are fully included. You are responsible for postage to the lab.

How long is the kit valid?

12 months from purchase.

Why We Partner with ZRT Laboratory

At Hormone Lab UK, every home test kit is analysed by ZRT Laboratory, a globally recognised, CLIA-certified diagnostic and research laboratory specialising in hormone testing, thyroid testing, fertility testing, adrenal function, neurotransmitter analysis, cardiometabolic health, heavy metals, and wellness testing. Founded in 1998 by renowned biochemist and breast cancer researcher Dr. David Zava, PhD, ZRT has become one of the world’s leading laboratories for advanced functional health testing.

With more than 11 million laboratory tests performed and healthcare professionals in over 96 countries relying on its expertise, ZRT is recognised for scientific excellence, analytical accuracy, and continuous innovation. The laboratory pioneered at-home saliva hormone testing, introduced the first commercially available dried blood spot (DBS) hormone tests, and developed Dried Urine Testing (DUTS), helping make advanced laboratory diagnostics more accessible and clinically meaningful.

Every sample is analysed using state-of-the-art LC-MS/MS and ICP-MS technology under rigorous quality control standards. ZRT collaborates with the CDC, NIH, and leading universities, supporting ongoing research and advances in laboratory medicine.