Test ID: SU-841 | Sample Type: Dried Urine + Saliva | Technology: Advanced LCMS analysis with specialist laboratory interpretation included | Laboratory: ZRT Laboratory (CLIA-certified) | Test Result: in 3-5 working days | Suitable For: Adults and Children | Collect Sample At Home: No Clinic Visit Required
This is an advanced functional assessment designed to evaluate the dynamic relationship between hormones and brain chemistry. This comprehensive profile measures 7 key sex and adrenal hormones in saliva using LCMS technology, alongside 42 dried urine neurotransmitters and diurnal hormone markers — delivering a total of 49 clinically relevant markers.
By integrating endocrine and neurotransmitter analysis, this test provides a detailed overview of hormonal balance, adrenal rhythm, stress response, mood regulation, and neurochemical pathways. It helps identify imbalances associated with anxiety, depression, ADHD, fatigue, sleep disturbances, and chronic stress.
The inclusion of diurnal cortisol and cortisone patterns offers deeper insight into daily adrenal function and stress physiology, delivering a data-driven and clinically meaningful neuroendocrine evaluation.
LCMS Saliva Hormone Tests
- Estradiol (E2)
- Estriol (E3)
- Estrone (E1)
- Progesterone (Pg)
- Testosterone (T)
- Dehydroepiandrosterone Sulfate (DS)
- Cortisol (C)
LCMS Dried Urine Neurotransmitter Tests
- Tryptophan (Trp)
- Serotonin (5-HT)
- 5-Hydroxyindoleacetic Acid (5-HIAA)
- Gamma-Aminobutyric Acid (GABA)
- Glycine (Gly)
- Taurine (Tau)
- Glutamate (Glu)
- Glutamine (Gln)
- Histidine (His)
- Histamine (HIST)
- N-Methylhistamine (N-MeHist)
- Phenylethylamine (PEA)
- Tyrosine (Tyr)
- Tyramine (Tyra)
- Dopamine (DA)
- Dihydroxyphenylacetic Acid (DOPAC)
- Homovanillic Acid (HVA)
- Norepinephrine (NE)
- Normetanephrine (NMN)
- Epinephrine (Epi)
- Vanillylmandelic Acid (VMA)
- Kynurenine (Kyn)
- Kynurenic Acid (KynAc)
- 3-Hydroxykynurenine (3OHKyn)
- Xanthurenate (Xanth)
- Creatinine (Crtn) ×5
- Free Cortisol (FC) ×4
- Free Cortisone (FCn) ×4
- 6-Sulfatoxymelatonin (MT6s) ×4
Not Sure Which Test Is Right for You?
Email us at info@hormonelab.co.uk with your gender, age, symptoms, medications, and any current treatments. We will forward your enquiry to our doctors and respond within 24 hours with a suggestion.
Why Combine Hormones & Neurotransmitters?
Hormones and neurotransmitters function as an interconnected network, constantly influencing one another. Chronic stress can disrupt cortisol and dopamine balance, oestrogen plays a role in serotonin production, progesterone modulates GABA activity, and DHEA and testosterone affect motivation, resilience, and mood. Evaluating both systems together provides a clearer understanding of the root causes behind symptoms such as fatigue, anxiety, mood instability, low libido, PMS, insomnia, and burnout.
The Benefit of Adding Free Cortisol, Free Cortisone & Creatinine
Including Free Cortisol, Free Cortisone, and Creatinine alongside neurotransmitter analysis enhances the clinical value of the assessment.
Comprehensive Neuroendocrine Insight
This combined approach evaluates both hormonal stress markers and neurotransmitter pathways, offering a broader understanding of neuroendocrine balance.
Stress Response Assessment
Free Cortisol and Free Cortisone reflect adrenal activity and daily stress adaptation, helping to identify dysregulation linked to chronic stress and mood disorders.
Mood & Cognitive Evaluation
By assessing stress hormones together with key neurotransmitters, the test clarifies how hormonal fluctuations may influence anxiety, depression, focus, and emotional resilience.
Adrenal Function Review
Cortisol and cortisone measurement supports evaluation of adrenal performance and potential dysfunction affecting both physical and mental health.
Sleep & Energy Regulation
Cortisol rhythm and neurotransmitter balance are central to healthy sleep patterns and stable energy levels.
Creatinine Normalisation
Creatinine ensures accurate interpretation of neurotransmitter and hormone levels by correcting for urine concentration variability.
Personalised Treatment Planning
The integrated results allow for more targeted clinical strategies, including nutritional, lifestyle, stress-management, and hormone-support interventions based on objective biochemical data.
Key Features
Comprehensive Neurochemical Profile
Evaluates a broad spectrum of neurotransmitters to provide detailed insight into overall brain chemistry and functional balance.
Objective Biomarker Measurement
Delivers precise, quantitative data to support clinical assessment and move beyond symptom-based evaluation.
Personalised Treatment Planning
Identifies specific imbalances to help guide tailored interventions, including medication optimisation, targeted nutritional support, and lifestyle strategies.
Clinical Diagnostic Support
Provides objective information that complements psychological and medical evaluations, assisting in the assessment of conditions such as anxiety, depression, ADHD, and other neurological or psychiatric disorders.
Targeted Therapeutic Direction
Highlights deficiencies or excesses within neurotransmitter pathways to enable focused and effective corrective strategies.
Treatment Monitoring
Allows practitioners to track biochemical changes over time and adjust interventions based on measurable progress.
Suitable for Adults and Children
Appropriate for both adult and paediatric use when clinically indicated.
Convenient At-Home Collection
Non-invasive dried urine sampling collected four times throughout the day, with simple storage and return.
Reliable Laboratory Analysis
Provides accurate, timely, and dependable results.
Delivery: Free delivery in the UK.
Shipping: Customers are responsible for shipping their samples to the laboratory.
No Additional Fee: The test kit includes a laboratory fee: no additional laboratory cost or tax.
How and when to collect your saliva samples: Saliva Testing Instructions
Click to see >> Sample Saliva Test Result Report
Click to see >> Advanced Neurotransmitter Test Report
The test must be used within 12 months after the purchase date.
Test Result Report: You will receive your test result via email within 3–5 working days after the laboratory receives your sample. Your report includes hormone levels in graphics and numbers with comments by a Specialist PhD. Dr on how to balance any imbalances and what to do next.
The Test Kit Pack Includes
- Test Requisition Form including Symptom Checklist
- Requisition Form to complete, including personal and medical history
- Collection instructions
- Urine strips for collecting dried urine samples and tubes for collecting saliva samples
- Instructions on how to use the Collection Kit
- Return Envelope
- Shipping instructions
NEUROTRANSMITTERS PLAY A KEY ROLE
Regulation of Mood and Emotions
Neurotransmitters such as serotonin, dopamine, and norepinephrine are central to emotional balance, motivation, and the experience of pleasure and reward. When these chemical messengers fall out of balance, symptoms associated with anxiety, depression, and bipolar disorder may develop.
Control of Motor Functions
Acetylcholine, dopamine, and GABA are essential for coordinating movement, muscle control, balance, and overall motor stability.
Cognitive Function and Learning
Glutamate, acetylcholine, and dopamine contribute to memory formation, attention, concentration, and information processing.
Regulation of Sleep and Wakefulness
Serotonin and norepinephrine help regulate circadian rhythm and sleep architecture.
Modulation of Pain Perception
Endorphins and enkephalins assist in reducing the perception of pain by moderating pain signal transmission.
Autonomic Functions
Neurotransmitters regulate heart rate, blood pressure, digestion, and respiration.
Regulation of Appetite and Satiety
Serotonin and related neuropeptides influence appetite, satiety, and food intake.
ARE YOU AFFECTED?
Mood Disorders
Altered levels of serotonin, dopamine, and norepinephrine are frequently associated with depression, anxiety disorders, and bipolar disorder.
Cognitive Impairment
Neurotransmitter dysregulation can impair memory, focus, and processing speed.
Sleep Disturbances
Imbalanced neurotransmitters may disrupt sleep-wake cycles, contributing to insomnia or excessive daytime fatigue.
Neurological Disorders
Dopamine disruption is associated with Parkinson's disease, while glutamate/GABA imbalances are linked to seizure activity.
Addiction and Substance Abuse
Dopamine dysregulation may increase susceptibility to addictive behaviours.
Hormonal Imbalances
Neurotransmitters influence endocrine function; persistent imbalances may disrupt hormone production.
Digestive Disorders
Low serotonin levels may contribute to IBS and other functional digestive conditions.
Neurotransmitters & Your Health
Anxiety and Depression
Imbalances in serotonin, norepinephrine, and dopamine are closely linked to anxiety and depressive disorders.
Sleep Disorders
Disturbances in serotonin and melatonin pathways can lead to difficulty falling or staying asleep.
Libido and Sexual Function
Dopamine plays a central role in sexual desire; imbalances may reduce libido or cause dysfunction.
Premenstrual Symptoms
Fluctuations in serotonin and GABA levels may intensify mood swings and emotional sensitivity before menstruation.
If these symptoms are present, an underlying neurotransmitter imbalance may be contributing. Objective testing can help identify root causes and guide personalised strategies.
Neurotransmitters & Their Impact
Neurotransmitters are chemical messengers that transmit signals between nerve cells, enabling continuous communication between the brain and the body. Balanced neurotransmitter activity supports stable mood, motivation, and mental clarity. Imbalances may contribute to depression, anxiety, bipolar disorder, schizophrenia, and other psychiatric conditions.
Can a Dysregulated Nervous System Impact Health?
A dysregulated nervous system can significantly impact overall health. Factors such as genetics, environmental stressors, chemical exposures, and nutritional deficiencies can disrupt neurotransmitter production. When this imbalance occurs, the nervous system attempts to compensate, but over time this may contribute to neurological or psychological symptoms. Objective testing helps identify root imbalances and supports more targeted and personalised intervention strategies.
Neurotransmitters & Symptoms
Do These Symptoms Sound Familiar?
If you regularly experience three or more of the following, a neurotransmitter imbalance may be contributing: ADD/ADHD, aggression, anxiety, fatigue, forgetfulness, hyperactivity, impulsive behaviours, insomnia, low libido, mood swings, OCD tendencies, panic attacks, poor concentration, PMDD, severe PMS, or persistent sleep problems.
Male Hormone-Related Symptoms
Male Estrogen/Progesterone Deficiency
May present with bone loss, depression, heart palpitations, hot flushes, neck or back pain, night sweats, and sleep difficulties.
Male Estrogen Dominance / Progesterone Deficiency
Can include irritability, cold body temperature, low libido, prostate concerns, urinary changes, and weight gain around the chest or hips.
Male Low Androgens (DHEA/Testosterone)
Common signs include fatigue, reduced stamina, low libido, decreased muscle mass, joint pain, irritability, depression, brain fog, headaches, skin thinning, rapid ageing, waist weight gain, and elevated triglycerides.
Male Low Cortisol
May involve low blood pressure, low blood sugar, fatigue, dizziness, joint pain, irritability, sugar cravings, and reduced stress tolerance.
Male High Cortisol
Often associated with anxiety, high blood pressure, sleep disturbance, depression, reduced muscle mass, central weight gain, skin thinning, and elevated triglycerides.
Female Hormone-Related Symptoms
Female Estrogen/Progesterone Deficiency
May include hot flushes, night sweats, depression, foggy thinking, vaginal dryness, infertility, incontinence, bone loss, sleep disturbance, tearfulness, and waist weight gain.
Female Estrogen Dominance / Progesterone Deficiency
Common signs include mood swings, irritability, anxiety, water retention, fibrocystic or tender breasts, headaches, uterine fibroids, bleeding changes, and hip weight gain.
Female Low Androgens (DHEA/Testosterone)
May present with fatigue, decreased libido, reduced muscle tone, bone loss, memory lapse, vaginal dryness, skin thinning, and reduced stamina.
Female High Androgens
Can involve acne, increased facial or body hair, scalp hair loss, irritability, waist weight gain, and elevated triglycerides.
Female Low Cortisol
Symptoms may include low blood pressure, low blood sugar, fatigue, fibromyalgia-like pain, slow pulse, stress intolerance, and sugar cravings.
Female High Cortisol
Often associated with anxiety, sleep disturbance, depression, memory issues, hot flushes, rapid ageing, bone loss, uterine fibroids, and central weight gain.
Testing & Collection
Saliva hormone testing measures cortisol, estrogen, progesterone, DHEA, and testosterone using a simple, non-invasive collection method. Samples are collected at home at specific times to ensure accurate hormone rhythm assessment.
Neurotransmitters and Children
Neurotransmitter imbalances can influence a child's emotional, cognitive, and physical development. Disruptions in serotonin, dopamine, acetylcholine, glutamate, and melatonin pathways have been linked to anxiety, ADHD, sleep issues, and developmental conditions such as autism spectrum disorder (ASD). Professional assessment and individualised treatment planning are essential for children.
Clinical Utility & Validity of Urinary Neurotransmitter Testing
Urinary neurotransmitter testing provides objective biochemical data that helps identify specific excesses or deficiencies, supporting more targeted and personalised treatment strategies rather than relying solely on trial-and-error approaches. Although urine testing is not a direct measurement of brain levels, urinary patterns often reflect systemic neurotransmitter activity and provide meaningful insight into overall neurochemical regulation.
Dried Urine – A Convenient Option
Dried urine collection is non-invasive and simple to perform at home. Four small samples are collected at specific times during the day using filter cards, then mailed to the laboratory. This method improves convenience, compliance, and practicality in clinical use.
Considerations
- The neurotransmitter test assumes proper kidney function. This test should not be used in individuals with compromised renal function.
- On the day of testing, restrict liquid intake to normal consumption to avoid dilute samples.
- Refrain from consuming alcohol, nicotine, bananas, pineapple, and walnuts on the day of testing as they may interfere with results.
