Test ID: S-142 | Sample Type: Saliva | Technology: LC-MS/MS | Results Time: 3–5 Working Days After Laboratory Receives Sample | Laboratory: ZRT Laboratory – CLIA Certified | Hormone Specialist Doctor Interpretation: Included | Suitable For: Adults and Children | Collect Sample At Home: No Clinic Visit Required
What Does This Test Measure?
Saliva — Advanced Male Hormone Panel (LC-MS/MS): Estrone (E1), Estradiol (E2), Estriol (E3), Testosterone, DHEAS, Progesterone, Morning Cortisol, Noon Cortisol, Evening Cortisol, Night Cortisol.
10 markers — the most comprehensive male saliva hormone panel available: combining the full oestrogen triad, androgenic status, adrenal reserve, and a complete 4-point diurnal cortisol curve in a single at-home kit.
Related Symptoms
Low libido or erectile dysfunction, persistent fatigue or low energy, loss of muscle mass or poor gym recovery, increased body fat particularly around the abdomen or chest, poor sleep or night waking, afternoon energy crashes, evening anxiety or racing thoughts, low mood, brain fog, reduced motivation, gynaecomastia or breast tenderness, water retention, burnout or overtraining, or symptoms of oestrogen excess with normal total testosterone on standard blood results.
Did You Know?
No standard GP blood panel measures all three oestrogens, free testosterone, DHEAS, progesterone, and the full daily cortisol curve simultaneously. This profile is the only way to assess in a single test whether oestrogen dominance is adipose-driven (elevated E1), whether testosterone-to-estradiol aromatisation is progesterone-mediated, whether adrenal depletion (DHEAS) is contributing to androgenic decline, and whether the cortisol rhythm at all four time points is suppressing testosterone synthesis, disrupting deep sleep recovery, or driving visceral fat accumulation. Each of these mechanisms operates independently — and each requires a distinct clinical response. This panel identifies which are active.
Who Should Consider This Test?
- Low libido or reduced sexual interest
- Erectile dysfunction or reduced sexual performance
- Persistent fatigue or low energy despite adequate sleep
- Loss of muscle mass or difficulty building strength
- Increased body fat, especially around the abdomen or chest
- Brain fog, poor concentration, or reduced motivation
- Low mood, irritability, anxiety, or depression
- Poor sleep quality, night waking, or insomnia
- Afternoon energy crashes or mid-day fatigue
- Evening anxiety, restlessness, or difficulty switching off
- Symptoms of andropause, typically from age 35+
- Gynaecomastia, breast tenderness, or water retention
- Burnout, overtraining, or sustained high physical or psychological stress
- Currently on TRT and wanting the most complete oestrogen, adrenal, and cortisol rhythm assessment
- Feeling hormonally off but told results are normal on standard blood tests
Why This Test Is Particularly Important for Men
- The full oestrogen triad (E1, E2, E3) reveals oestrogen source and metabolic conversion — elevated estrone relative to estradiol indicates adipose-driven aromatisation; combined with the cortisol curve, it identifies whether cortisol-driven visceral fat accumulation is the primary aromatase driver
- The 4-point cortisol curve reveals how HPA axis function shapes testosterone across the full day — elevated noon cortisol drives visceral fat and insulin resistance; elevated evening cortisol suppresses progesterone and testosterone precursor synthesis; elevated night cortisol directly suppresses the deep sleep phases responsible for the majority of daily testosterone production
- DHEAS is the primary adrenal testosterone precursor — its inclusion alongside the full cortisol curve reveals whether adrenal depletion is contributing to androgenic decline and whether cortisol-mediated DHEAS suppression is the mechanism
- Progesterone is the natural aromatase inhibitor in men — low progesterone measured alongside the full oestrogen triad identifies whether aromatase dysregulation is progesterone-mediated and whether progesterone support is the appropriate clinical priority
- The cortisol-to-testosterone ratio across the full day is the most clinically informative marker of overtraining, adrenal-driven androgenic suppression, and burnout — a pattern invisible to any single time-point measurement
Not Sure Which Test Is Right for You?
Email us at info@hormonelab.co.uk with your gender, age, symptoms, medications, and any current treatments. We will forward your enquiry to our doctors and respond within 24 hours.
How It Works
- Order your kit — delivered directly to your door
- Collect four saliva samples across the day — morning (7–9am, before eating or drinking), noon, evening, and night — following the detailed instructions provided
- Post your samples to ZRT Laboratory using the return instructions provided
- Receive your full report by email within 3–5 working days
What You Receive
- Estrone (E1), Estradiol (E2), Estriol (E3), Testosterone, DHEAS, Progesterone, and 4-point Cortisol results with male-specific reference ranges
- Full diurnal cortisol curve graph and oestrogen triad chart
- Full oestrogen triad ratio analysis, testosterone-to-estradiol assessment, cortisol-to-testosterone ratio, and adrenal–androgenic interaction evaluation by a Hormone Specialist PhD Doctor
- Personalised next-step recommendations
- Laboratory fee included — no hidden charges
Why We Use ZRT Laboratory
All testing is performed by ZRT Laboratory — a globally recognised, CLIA-certified diagnostic laboratory founded in 1998 by Dr. David Zava, PhD. ZRT has performed over 11 million tests and is trusted by healthcare professionals in 96+ countries.
- Pioneers of at-home dried blood spot (DBS) and saliva hormone testing
- Advanced LC-MS/MS technology for clinical-grade accuracy
- Qualified for the CDC Hormone Standardization (HoSt) Program
- Personalised reference ranges based on age and sex
Through our partnership with ZRT Laboratory, Hormone Lab UK delivers internationally recognised, research-backed hormone testing to people across the UK and Europe — from the comfort of home.
Frequently Asked Questions
What hormones does this test measure?
Estrone (E1), Estradiol (E2), Estriol (E3), Testosterone, DHEAS, Progesterone, and Cortisol at four time points (morning, noon, evening, night) — 10 markers covering the complete male hormonal and HPA axis picture.
When should I collect my samples?
Four saliva samples are collected across the day: morning (ideally 7–9am, before eating or drinking), noon, evening, and night. Full collection instructions are included in your kit.
Can I use this test if I am on TRT?
Yes. This is the most complete panel available for men on TRT, providing the full oestrogen triad, DHEAS adrenal reserve, and the complete cortisol rhythm alongside testosterone — everything needed to guide aromatase management, adrenal support, and therapy optimisation.
How does this differ from Male Saliva Hormone Profile III?
Profile III measures testosterone, DHEAS, progesterone, morning cortisol, and the full oestrogen triad — 7 markers at a single morning time point. The Advanced Profile extends this with a full 4-point diurnal cortisol curve — providing complete visibility of how cortisol behaves throughout the day and its interaction with testosterone synthesis, recovery, and adrenal regulation.
Does the price include the laboratory fee?
Yes. The full laboratory analysis fee is included. There are no additional charges. You are only responsible for posting your samples to the laboratory.
How long is the kit valid for?
The kit must be used within 12 months of the purchase date.
