Female Saliva Hormone Profile III (LC-MS/MS)

Test ID: S-125 | Sample Type: Saliva | Technology: LC-MS/MS | Results Time: 3–5 Working Days After Laboratory Receives Sample | Laboratory: ZRT Laboratory – CLIA Certified | Hormone Specialist Doctor Interpretation: Included | Suitable For: Adults and Children | Collect Sample At Home: No Clinic Visit Required

What Does This Test Measure?

Saliva — Advanced Female Hormone Panel (LC-MS/MS): Estrone (E1), Estradiol (E2), Estriol (E3), Progesterone, Testosterone, DHEAS, Cortisol.
7 markers — the most complete single-point oestrogen assessment available in a female saliva panel, combining the full oestrogen triad with progesterone balance, androgen sufficiency, adrenal reserve, and morning cortisol in a single at-home kit.

Related Symptoms

Irregular, heavy, or painful periods, PMS including mood swings, bloating or breast tenderness, perimenopausal or menopausal symptoms including hot flushes and night sweats, low libido, persistent fatigue or low energy despite adequate sleep, anxiety or emotional instability, poor sleep quality or night waking, weight gain particularly around the abdomen, fertility concerns, burnout or chronic stress, or feeling hormonally off despite normal standard blood test results.

Did You Know?

Most female hormone panels measure only estradiol (E2). This profile adds estrone (E1) and estriol (E3), completing the oestrogen triad. In women, estrone becomes the dominant oestrogen after menopause and is primarily produced by adipose tissue via aromatase conversion — making it the key marker of postmenopausal oestrogenic activity. Estriol, by contrast, is the most protective of the three oestrogens and its ratio relative to estradiol and estrone provides clinically meaningful information about oestrogenic balance that E2 alone cannot reveal. The E1:E2:E3 ratio guides decisions about whether oestrogenic support, progesterone supplementation, or adrenal optimisation is the appropriate clinical priority — a level of resolution unavailable from standard hormone panels.

Who Should Consider This Test?

• Irregular, heavy, or painful periods
• PMS — mood swings, bloating, breast tenderness, or irritability
• Perimenopausal or menopausal symptoms — hot flushes, night sweats, brain fog, or low mood
• Low libido or reduced sexual responsiveness
• Persistent fatigue or low energy despite adequate sleep
• Anxiety, depression, or emotional instability
• Difficulty sleeping or waking in the night
• Weight gain or difficulty losing weight, especially around the abdomen
• Fertility concerns or difficulty conceiving
• Symptoms of oestrogen dominance — bloating, breast tenderness, or heavy bleeding
• Postmenopausal women wanting a full oestrogenic status assessment
• Burnout, chronic stress, or emotional exhaustion
• Currently on HRT or bioidentical hormones and wanting a complete oestrogen triad alongside adrenal markers
• Feeling hormonally off but told results are normal on standard blood tests

Why This Test Is Particularly Important for Women

• The full oestrogen triad (E1, E2, E3) reveals oestrogenic source and metabolic balance in women — elevated estrone relative to estradiol indicates adipose-driven aromatisation; a low estriol-to-estradiol ratio indicates a more proliferative oestrogenic environment; these patterns guide HRT selection, progesterone dosing, and lifestyle prioritisation
• Saliva LC-MS/MS measures free, bioavailable estradiol and testosterone — the fractions actually driving symptoms; standard blood immunoassay measures total hormone including the protein-bound inactive fraction, which frequently masks clinically significant imbalances in women on HRT or with elevated SHBG
• The progesterone-to-estradiol ratio is the most clinically meaningful number in this panel — oestrogen dominance drives the majority of PMS, cycle irregularity, breast tenderness, and perimenopausal anxiety even when absolute estradiol appears within range
• DHEAS is the adrenal precursor to both oestrogen and testosterone in women — its inclusion reveals whether adrenal depletion is driving sex hormone decline independently of ovarian function
• Cortisol provides the HPA axis context — elevated morning cortisol suppresses progesterone via pregnenolone steal, depletes DHEAS, and promotes visceral fat-driven aromatase activity, compounding both oestrogen dominance and androgenic insufficiency

Not Sure Which Test Is Right for You?

Email us at info@hormonelab.co.uk with your gender, age, symptoms, medications, and any current treatments. We will forward your enquiry to our doctors and respond within 24 hours.

How It Works

1. Order your kit — delivered directly to your door
2. Collect your saliva sample at home following the detailed instructions — ideally on Day 19–21 of your cycle (7 days after ovulation) for optimal progesterone and estradiol capture; guidance for postmenopausal and HRT users is included
3. Post your sample to ZRT Laboratory using the return instructions provided
4. Receive your full report by email within 3–5 working days

What You Receive

• Estrone (E1), Estradiol (E2), Estriol (E3), Progesterone, Testosterone, DHEAS, and Cortisol results with female life-stage-specific reference ranges
• Visual charts for all seven hormones
• Full oestrogen triad ratio analysis, progesterone-to-estradiol assessment, and adrenal–sex hormone interaction evaluation by a Hormone Specialist PhD Doctor
• Personalised next-step recommendations
• Laboratory fee included — no hidden charges

Why We Use ZRT Laboratory

All testing is performed by ZRT Laboratory — a globally recognised, CLIA-certified diagnostic laboratory founded in 1998 by Dr. David Zava, PhD. ZRT has performed over 11 million tests and is trusted by healthcare professionals in 96+ countries.

• Pioneers of at-home dried blood spot (DBS) and saliva hormone testing
• Advanced LC-MS/MS technology for clinical-grade accuracy
• Qualified for the CDC Hormone Standardization (HoSt) Program
• Personalised reference ranges based on age and sex

Through our partnership with ZRT Laboratory, Hormone Lab UK delivers internationally recognised, research-backed hormone testing to people across the UK and Europe — from the comfort of home.

Frequently Asked Questions

What hormones does this test measure?
Estrone (E1), Estradiol (E2), Estriol (E3), Progesterone, Testosterone, DHEAS, and Cortisol — 7 markers providing the most complete female oestrogen and hormonal panel available from a single at-home saliva test.

When should I collect my sample during my menstrual cycle?
Day 19–21 of your cycle (approximately 7 days after ovulation) is optimal for capturing progesterone and estradiol in context. Full timing guidance for irregular cycles, perimenopause, postmenopause, and HRT users is included in your kit.

How does this differ from Female Saliva Hormone Profile I?
Profile I measures estradiol, progesterone, testosterone, DHEAS, and morning cortisol — 5 markers. Profile III extends this by adding estrone (E1) and estriol (E3), providing the complete oestrogen triad for a full assessment of oestrogenic source, metabolic balance, and postmenopausal hormonal status.

Can I use this test if I am postmenopausal or on HRT?
Yes. This test is particularly valuable for postmenopausal women and those on HRT wanting the most complete oestrogenic picture to guide therapy decisions and identify whether adrenal or ovarian dysfunction is the primary driver of residual symptoms.

Does the price include the laboratory fee?
Yes. The full laboratory analysis fee is included. There are no additional charges. You are only responsible for posting your sample to the laboratory.

How long is the kit valid for?
The kit must be used within 12 months of the purchase date.